Meso scale discovery assays provide a unique immunoassay platform for the detection of analytes in biological samples. MSD mesoscale offers around 400 unique assay kits along with customization assay capabilities. Such a wide range of assaying capabilities has empowered researchers to make precise and accurate estimations of analytes in different biological matrices.
Today almost all clinical research organizations, pharmaceutical and biotech companies, and academic and government organizations have adopted meso scale discovery assays in routine analysis. Patterned arrays are combined with meso scale discovery electrochemiluminescence to provide parallel analysis of multiple biological analytes. There are five major types of MSD assay kits, namely R-Plex, U-Plex, T-Plex, V-Plex, and S-Plex assay kits. Let us explore each of these assay kits in detail.
R-Plex MSD assay kits
R-Plex assays are matched antibody sets capable of performing singleplex and multiplex assays. They provide an easy and rapid way of designing immunoassays that can deliver all the characteristics of an MSD multi-array platform. Each mesoscale assay kit comprises a detection antibody labeled with SULFO-TAG, biotinylated capture antibody, and calibrator. R-Plex singleplex assays consist of streptavidin plates with MSD Gold spots whereas multiplex assays are a combination of U-Plex plates and R-Plex antibody units.
U-Plex MSD assay kits
With more than 200 assays available, the U-Plex MSD assay is a reliably flexible and customized multiplexing platform. U-Plex assay kits are standalone kits or can be combined with own assay parameters empowering researchers and scientists to come up with personalized multiplex combinations.
T-Plex MSD assay kits
T-Plex kits are capable of simultaneous protein level analysis in a single assay format. They are an ideal choice for rapid and convenient measuring of biomarker assay levels in small-volume samples. T-Plex assay kits are available in single and multiplex format where they are grouped as ultra-sensitive, tissue culture, serum/plasma, whole-cell lysate, and base kits.
V-Plex MSD assay kits
V-Plex assay platforms provide reliable, reproducible, and confident assay results through thoroughly validated analytical methods with well-defined performance specifications. V-Plex assays kits are found in both single and multiplex panels, offering high sensitivity, a wide detection range, and consistency.
S-Plex MSD assay kits
S-Plex MSD is an ultra-sensitive assay kit. S-Plex assay platform can reduce the lower limit of detection up to 1000 times compared to other assay formats. Low femtogram/ml level detection is easily achieved through S-Plex assay kits. Such lower detection limits mean researchers have to use less sample volume, critical reagents, and concentrations in bioanalytical assays. S-Plex assay formats are easy to use as they leverage the same procedure and expertise of other MSD assay platforms.
Mesoscale assay’s unique spot patterns enable single and multiplex detection of analytes in samples. MSD mesoscale offers a wide range of immunoassay formats such as MSD ELISA with crucial assay characteristics that include minimal sample usage, ultra-low detection, and linear dynamic range of up to five logs. Such features help MSD mesoscale perform analyte detection in crude samples without the need for multiple dilutions. Further advancements in its features will only skyrocket its use in the clinical and drug development domain.